Random Sequencing of Paramecium Somatic DNA
نویسندگان
چکیده
منابع مشابه
Random sequencing of Paramecium somatic DNA.
We report a random survey of 1 to 2% of the somatic genome of the free-living ciliate Paramecium tetraurelia by single-run sequencing of the ends of plasmid inserts. As in all ciliates, the germ line genome of Paramecium (100 to 200 Mb) is reproducibly rearranged at each sexual cycle to produce a somatic genome of expressed or potentially expressed genes, stripped of repeated sequences, transpo...
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positive transformant clones probed with the PcVenus sequence revealed that the injected DNA had become randomly inserted into the chromosome. Free linear monomers or multimers of injected DNA molecules as seen in P. primaurelia and P. tetraurelia were not evident even though the plasmid possessed extant telomeric repeats at its both extremities. We also cloned fragments containing the integrat...
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A consortium of laboratories undertook a pilot sequencing project to gain insight into the genome of Paramecium. Plasmid-end sequencing of DNA fragments from the somatic nucleus together with similarity searches identified 722 potential protein-coding genes. High gene density and uniform small intron size make random sequencing of somatic chromosomes a cost-effective strategy for gene discovery...
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Next Generation Sequencing (NGS) technology is based on cutting DNA into small fragments, and their massive parallel sequencing. The multiple overlapping segments termed "reads" are assembled into a contiguous sequence. To reduce sequencing errors, every genome region should be sequenced several dozen times. This sequencing approach is based on the assumption that genomic DNA breaks are random ...
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Paramecium, like other ciliates, remodels its entire germline genome at each sexual generation to produce a somatic genome stripped of transposons and other multicopy elements. The germline chromosomes are fragmented by a DNA elimination process that targets heterochromatin to give a reproducible set of some 200 linear molecules 50 kb to 1 Mb in size. These chromosomes are maintained at a ploid...
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ژورنال
عنوان ژورنال: Eukaryotic Cell
سال: 2002
ISSN: 1535-9778,1535-9786
DOI: 10.1128/ec.1.3.341-352.2002